Adaptive harmonic generation microscopy of mammalian embryos.
نویسندگان
چکیده
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.
منابع مشابه
Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.
We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, su...
متن کاملAn adaptive method for accurate sharing of harmonic and unbalanced loads in islanded microgrids
In this paper, a method is proposed for accurate sharing of harmonic and unbalanced loads in islanded microgrids. The proposed method can adaptively improve the load sharing among distributed generation (DG) units based on the circulating currents rules and harmonic and unbalanced powers provided by each DG. Compared with previous methods, the proposed method does not require a dedicated centra...
متن کاملImaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy.
In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex d...
متن کاملCombined third-harmonic generation and four-wave mixing microscopy of tissues and embryos
Nonlinear microscopy can be used to probe the intrinsic optical properties of biological tissues. Using femtosecond pulses, third-harmonic generation (THG) and four-wave mixing (FWM) signals can be efficiently produced and detected simultaneously. Both signals probe a similar parameter, i.e. the real part of the third-order nonlinear susceptibility χ((3)). However THG and FWM images result from...
متن کاملIn vivo developmental biology study using noninvasive multi-harmonic generation microscopy.
Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics letters
دوره 34 20 شماره
صفحات -
تاریخ انتشار 2009